High-resolution liquid chromatographic separations in 400 nm deep micro-machined silicon channels and fluorescence charge-coupled device camera detection ... [An article from: Analytica Chimica Acta]
 
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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We report on a study exploring the possibilities of silicon etched, flat-rectangular nano-channels for the acceleration of liquid chromatography separations. Using the shear-forces originating from a moving channel part to propagate the mobile phase flow, a mixture of two neutral coumarin dyes could be separated in less than 50ms and with a resolution of over N=10^6plates/m. The obtained experimental van Deemter-plot show a good agreement with the theoretical expectations and has a minimal plate height around H=1.0@mm (k=1.5) and H=0.6@mm (k=0.5). The different steps in the fabrication of the nano-channels, and the advantages of a novel detection scheme, based on fluorescent charge-coupled device-array imaging under stopped-flow conditions, are discussed as well.